Specific Oligonucleotide Primers for Detection of Lecithinase-Positive Bacillus spp. by PCR
نویسندگان
چکیده
منابع مشابه
Specific oligonucleotide primers for detection of endoglucanase positive Bacillus subtilis by PCR
A polymerase chain reaction (PCR) assay was developed for discrimination of Bacillus subtilis from other members of B. subtilis group as well as rapid identification from environmental samples. Primers ENIF and EN1R from endoglucanase gene were used to amplify a1311 bp DNA fragment. The specificity of the primers was tested with seven reference strains and 28 locally isolated strains of endoglu...
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When Bacillus anthracis is grown in egg yolk medium, a reaction occurs which is presumably due to lecithinase (Colmer, 1948; McGaughey and Chu, 1948; Chu, 1949). In liquid egg yolk medium the lecithinase reaction is characterized by the formation of an opalescent suspension and flocculation of particles which rise to the top of the medium as a curd. A test such as this is usually referred to as...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1995
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.61.6.2452-2452.1995